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KMID : 0613820080180040482
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Journal of Life Science
2008 Volume.18 No. 4 p.482 ~ p.487
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Screening and Identification of Alkaline Protease Inhibitor-Producing Marine-derived Actinomyces
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Kang Sung-Il
Kong Jai-Yul
Choi Yeung-Joon
Kim Min-Yong
Son Hong-Joo
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Abstract
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In this study, we screened and identified the bacterial strain showing high alkaline pretense inhibitor activity from marine environment. Nine bacterial strains with alkaline pretense inhibitor activity were isolated from marine sediments. Among them, strain C12 had the highest alkaline pretense inhibitor activity and was selected for further taxonomical study. On the basis of morphological and physiological characteristics, strain C12 was identified as the genus Streptomyces. A phylogenetic analysis of the 165 rDNA showed that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces thermocarboxydus. Morphological characteristics showed cylindrical spore chain and smooth spore surface by scanning electron microscope. Strain C12 was grown on all media except for ISP 9 agar. This strain could be grown in the medium containing up to 9% NaCl.
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KEYWORD
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Marine actinomycete, protease inhibitor, Streptomyces sp.
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